Over 300 analysts and researchers from pharmaceutical and nutraceutical industry agreed with us at the 16th Waters Users Meeting that proper vitamin analysis is crucial for quality assurance of the products and confirmation of the labelled content.
Analysis of Fat-Soluble and Water-Soluble Vitamins in Food Supplements by using QDa MS Detection
By addressing the need for content determination of active ingredients in food supplements and the analytical challenges related to it, we triggered great interest in the audience from both dietary supplements and pharmaceutical industry. Our presentation focused on solving the complex analytical questions by Analysis of Fat-Soluble and Water-Soluble Vitamins in Food Supplements by using QDa MS Detection.
Vitamin fortification of food and beverage products is an increasing trend, which has come about due to the poor levels of nutrients in many people’s diets. There are a lot of different products on the market (dietary supplements, foods, beverages) that include vitamins as active compounds. As pharmaceuticals, food supplements need to be tested to be trusted for quality and offer the best product to the customers.
Analytical challenge: assure the quality of complex multivitamin products
The purpose of the vitamin analysis is to assure the quality of the products and confirm the labelled content. The main challenges are related to the various structures and different nature of the vitamins including analytes that lack chromophore or are present at very low concentration.
Moreover, the multivitamin products represent very complex samples that need specific approach for both sample preparation and analysis because here the vitamins are present across a very wide concentration range. Dealing with such samples is challenging and time consuming.
UHPLC method with mass detection by using a QDa detector
To date there is no HPLC-UV analytical method that is able to quantify all vitamins of interest present in the recommended daily intake in multivitamin products.
Therefore, we need a more selective and sensitive method to satisfy our analytical needs. By developing a UHPLC method with mass detection by using a QDa detector we achieved:
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- Simultaneous analysis of all main vitamins in their most commonly used forms, short run time and improved lab turnaround time.
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- Quantification at low concentrations (RDA doses) of the most problematic vitamins (B7, B12 and D3) and sensitivity as well as selectivity.
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- Quantification of all vitamins in wide analytical ranges in complex matrices.
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- User friendly, sensitive, selective and robust method appropriate for routine analysis.
16th Waters Users Meeting
Labtim and Waters Corporation organised a traditional meeting that attracts the best industry professionals. Ranging from the latest trends to the most advanced analytical methods, the meeting presents an opportunity to learn and meet. INSLAB’s team is proud and grateful to the Labtim and Waters company for providing this important yearly event to our community.
Tags: Labtim, QDa, UHPLC, vitamin analysis, Waters